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4-Cholesten-3-one is used as a chiral building block for proteomics research. It is an intestinal metabolite of cholesterol and exhibits an anti-obesity effect on animals.
Benesch, M. G. K.; Mannock, D. A.; Lewis, R. N. A. H.; McElhaney, R. N. A DSC and FTIR spectroscopic study of the effects of the epimeric 4-cholesten-3-ols and 4-cholesten-3-one on the thermotropic phase behaviour and organization of dipalmitoylphosphatidylcholine bilayer membranes: Comparison with their 5-cholesten analogues. Chem. Phys. lipids 2014, 177, 71-90.
Kim, J.; London, E. Using Sterol Substitution to Probe the Role of Membrane Domains in Membrane Functions. Lipids 2015, 50 (8), 721-734.