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MES monohydrate is a biological buffer often referred to as Goods buffer. It is useful for cell culture media and protein based buffer formulations to maintain a stable environment in solution. It is also used in the purification bioprocesses of antibodies, peptides, proteins, blood components and growth factors. It is a useful resolving agent for very small proteins on bis-tris gels. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.
Biological buffer, pKa = 6.15 at 20o: Biochemistry, 5, 467 (1966).
Rahman, R. N. Z. R. A.; Masomian, M.; Leow, A. T. C.; Ali, M. S. M. Influence of protein solution in nucleation and optimized formulation for the growth of ARM lipase crystal. J. Cryst. Growth 2015, 426, 234-242.
Baumann, P.; Baumgartner, K.; Hubbuch, J. Influence of binding pH and protein solubility on the dynamic binding capacity in hydrophobic interaction chromatography. J. Chromatogr. A 2015, 1396, 77-85.
Gefahrenhinweise (EU): H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.