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Used to formulate CAPS buffer, a zwitterionic buffer useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electro transfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. and minimal reactivity with enzymes or proteins, minimal salt effects.
Jelena Zaitseva.; I Barry Holland.; Lutz Schmitt. The role of CAPS buffer in expanding the crystallization space of the nucleotide-binding domain of the ABC transporter haemolysin B from Escherichia coli.Acta Crystallogr D Biol Crystallogr.2004,60(6), 1076-1084.
Daniele de Sanctis.; Sylvia Dewilde.; Clemens Vonrhein.; Alessandra Pesce.; Luc Moens.; Paolo Ascenzi.; Thomas Hankeln.; Thorsten Burmester.; Marco Ponassi.; Marco Nardini.; Martino Bolognesi. Bishistidyl heme hexacoordination, a key structural property in Drosophila melanogaster hemoglobin.J Biol Chem.2005,280,(29), 27222-27229.
Buffer, pKa = 10.40 at 20o: Biochemistry, 5, 467 (1966).
Gefahrenhinweise (EU): H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.