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O-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride is used in the preparation of oximes of steroids having keto group. It is used as a derivatization reagent in the determination of thromboxane B2, prostaglandins, amygdalin and a variety of aldehydes, ketones and acids. It is a versatile reagent utilized for the detection of carbonyl-containing compounds by gas chromatography (GC), gas chromatography mass spectrometry (GC-MS) and other methods. As a derivatization reagent, it is also used for EC/LC analysis of keto-steroids and carbohydrates.
Very sensitive reagent for derivatization of steroids for GC: J. Chromat. Sci., 13, 97 (1975); J. Chromat., 114, 81 (1975).
Rodigast, M.; Mutzel, A.; Iinuma, Y.; Haferkorn, S.; Herrmann, H. Characterisation and optimisation of a sample preparation method for the detection and quantification of atmospherically relevant carbonyl compounds in aqueous medium. Atmos. Meas. Tech. 2015, 8 (6), 2409-2416.
Zamora, R.; Navarro, J. L.; Aguilar, I.; Hidalgo, F. J. Lipid-derived aldehyde degradation under thermal conditions. Food Chem. 2015, 174, 89-96.