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It is employed as an electrophoresis tracking dye and also finds its uses as an industrial dye, a laboratory acid-base indicator and a biological stain to detect proteins and nucleic acids (absorbance at 610 nm), particularly when staining living tissues. In gel electrophoresis it can be used as a color marker and as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.
Menzies DW. Bromphenol blue as a stain for muscle striations.Stain Technol.1961,36285-287.
Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue.Anal Biochem.,1978,88(2), 605-11.
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