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7240-90-6 - 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+% - X-gal - B21034 - Alfa Aesar

B21034 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

CAS-Nummer
7240-90-6
Synonyme
X-gal

Größe Preis ($) Menge Verfügbarkeit
10mg 29,60
50mg 60,20
250mg 194,00
Zum Warenkorb hinzufügen Zur Angebotsanfrage hinzufügen Artikel anzeigen

5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

MDL
MFCD00005666
EINECS
230-640-8

Chemische Eigenschaften

Formel
C14H15BrClNO6
Molmasse
408.64
Schmelzpunkt
ca 236° dec.
Sensitivität
Moisture & Light Sensitive
Löslichkeit
Soluble in 100 mM in DMSO.

Anwendungen

It is a substrate for beta-galactosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. It is also used to detect activity of this enzyme in histochemistry and bacteriology. It is employed as a colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds. They are used in biochemical assays and in diagnosis as indicators, particularly in the form of enzyme substrates. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Bemerkungen

Moisture & Light sensitive. Store away from oxidizing agents, heat, water/moisture and light. Store under inert gas. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Literaturverweise

Christiaen L, et al. X-gal staining of electroporated sea squirt (Ciona) embryos.Cold Spring Harbor protocols.20092009(12), prot5346.

Kim MY, et al. X-gal inhibits the swarming of Vibrio species.J. Microbiol. Methods.200666(3), 552-555.

Weitere Referenzen

Beilstein
1402009
Harmonized Tariff Code
2934.99
TSCA
No

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