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N-Hydroxysulfosuccinimide sodium salt is used as crosslinking agents for preparing hydrophilic active esters. It is also used to activate carboxylic acids to the more reactive succinimidyl esters for coupling, biotinylation and conjugation with fluorophores. Further, it acts as a hydrophilic ligand and also used in covalently binding proteins to carboxyl containing beads, resins, other nanoparticles. It is involved in the conversion of carboxyl groups to primary amine-reactive sulfo-NHS esters.
Neves, M. M. P. S.; González-García, M. B.; Hernández-Santos, D.; Fanjul-Bolado, P. Streptavidin functionalized nickel nanowires: A new ferromagnetic platform for biotinylated-based assays. Talanta 2015, 144, 283-288.
Huang, X.; Xu, Z.; Mao, Y.; Ji, Y.; Xu, H.; Xiong, Y.; Li, Y. Gold nanoparticle-based dynamic light scattering immunoassay for ultrasensitive detection of Listeria monocytogenes in lettuces. Biosens. Bioelectron. 2015, 66, 184-190.s