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3,3'-Diaminobenzidine is used as peroxidase substrate and a reagent for spectrophotometric determination of selenium. DAB is used for the immunohistochemical staining of nucleic acids and proteins. As a dark brown dye, was used as an antibody-specific stain to identify the paired antibodies in breast tissue. It may be used for the synthesis of a useful, linear, Schiff-base coordination polymer.
N.H.Chua. Photooxidation of 3,3'-diaminobenzidine by blue-green algae and Chlamydomonas reinhardii. Biochimica et Biophysica Acta. 1972, 267, (1),179-189
Volker Herzog; H.Dariush Fahimi. A new sensitive colorimetric assay for peroxidase using 3,3?-diaminobenzidine as hydrogen donor. Analytical Biochemistry. 1973, 55, (2),554-562
Gefahrenhinweise (EU): H341-H350
Suspected of causing genetic defects. May cause cancer.
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