I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.
4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies. It is also used for N-terminal modification of peptides in automated synthesis.
Desmond J O'Shea; Tomas C O'Riordan; Paul J O'Sullivan; Dmitri B.Papkovsky. Homogeneous time-resolved fluorescence assays for the detection of activity and inhibition of phosphatase enzymes employing phosphorescently labeled peptide substrates. Analytica Chimica Acta. 2007, 583,(2), 349-356.
Tomas C O'Riordan; James Hynes; Dmitri Yashunski; Gelii V.Ponomarev; Dmitri B.Papkovsky. Homogeneous assays for cellular proteases employing the platinum(II)-coproporphyrin label and time-resolved phosphorescence. Analytical Biochemistry. 2005, 342,(1), 111-119.