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Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into F(ab')2 or Fab fragments. Ficin acts on bonds involving uncharged and/or aromatic amino acids. The immobilization of ficin enhances stability against denaturation, heat and autolysis. Immobilization also eliminates any potential for antibody-enzyme adducts that cause continued sample digestion. In addition to these advantages, immobilization allows the ficin to be reused.
M Mariani; M Camagna; L Tarditi; E Seccamani. A new enzymatic method to obtain high-yield F(ab)2 suitable for clinical use from mouse IgGl. Molecular Immunology, 1991, 28(1-2), 69-77.
Filippova IYu; E N Lysogorskaya; E S Oksenoit; G N Rudenskaya; V M Stepanov. L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide--a chromogenic substrate for thiol proteinase assay. Analytical Biochemistry, 1984,143,(2), 293-297.
Gefahrenhinweise (EU): H315-H319-H334-H335
Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.
Do not breathe dust/fume/gas/mist/vapours/spray. Obtain special instructions before use. Wear protective gloves. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.