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This buffer is widely used in molecular biology, biochemistry and chromatography. Useful in conjunction with Sodium Phosphate, dibasic in the preparation of biological buffers. Used in many applications including the purification of antibodies. It is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
J. C. Hoogvliet; M. Dijksma; B. Kamp and W. P. van Bennekom. Electrochemical Pretreatment of Polycrystalline Gold Electrodes To Produce a Reproducible Surface Roughness for Self-Assembly:? A Study in Phosphate Buffer pH 7.4. Anal. Chem.2000, 72 (9), 2016-2021.
Henrik Vorum; Rolf Brodersen; Ulrich Kragh-Hansen; Anders O. Pedersen. Solubility of long-chain fatty acids in phosphate buffer at pH 7.4. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1992, 1126 (2), 135-142.