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PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.
P. S. Baur,; T. R. Stacey.The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy. Journal of Microscopy. 1977 , 109 (3), 315-327.
K. Yamamoto,; K. Ogawa. Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline phosphatase activity in rat hepatocytes. Histochemistry. 1983, 77 (3), 339-351.