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5625-37-6 - PIPES, 1.0M buffer soln., pH 6.5 - J60659 - Alfa Aesar

J60659 PIPES, 1.0M buffer soln., pH 6.5

CAS-Nummer
5625-37-6
Synonyme

Größe Preis ($) Menge Verfügbarkeit
250ml 55,65
500ml 81,00
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PIPES, 1.0M buffer soln., pH 6.5

MDL
MFCD00006159

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Fully misicible in water. Soluble in water (0.05 M), giving a clear, colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.

Anwendungen

PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.

Bemerkungen

Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents, bases, reducing agents, heat.

Literaturverweise

P. S. Baur,; T. R. Stacey.The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy. Journal of Microscopy. 1977 , 109 (3), 315-327.

K. Yamamoto,; K. Ogawa. Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline phosphatase activity in rat hepatocytes. Histochemistry. 1983, 77 (3), 339-351.

Weitere Referenzen

Harmonized Tariff Code
2933.59
TSCA
Yes

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