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Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.
RH Aebersold.; J Leavitt.; RA Saavedra. Internal amino acid sequence analysis of proteins separated by one-or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. . PNAS. 1987, 84,(20), 6970-6974.
MA Paz, R Flückiger, A Boak, HM Kagan. Specific detection of quinoproteins by redox-cycling staining. The Journal of Biological Chemistry.1991, 266, 689-692.
Gefahrenhinweise (EU): H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.