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HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2?-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydrogen-ion buffering of algal cultures. Separation of human platelets from plasma proteins including factor VIII VWF by a combined albumin gradient-gel filtration method by using HEPES buffer. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions.
Charles Shipman Jr. Evaluation of 4-(2-Hydroxyethyl)-1-piperazineëthanesulfonic Acid (HEPES) as a Tissue Culture Buffe. Exp Biol Med (Maywood) January 1969. 1969, 130 (1), 305-310.
G. I. McFadden,; M. Melkonian. Use of Hepes buffer for microalgal culture media and fixation for electron microscopy. Journal name. 1986, 25 (4), 551-557..