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NP-40 lysis buffer - J60766 - Alfa Aesar

J60766 NP-40 lysis buffer

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
250ml 39,80
500ml 63,60
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NP-40 lysis buffer

Chemische Eigenschaften

Form
Liquid
Löslichkeit
It is soluble in water.

Anwendungen

NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions.

Bemerkungen

This buffer contains: 50mM Tris-HCl (pH 7.4), 150mM NaCl, 1%NP-40 and 5mM EDTA. To use, incubate cells in 5 volumes of buffer on ice for 10-30 minutes.

Literaturverweise

M Herrmann.; HM Lorenz.; R Voll.; M Grünke. A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res. 1994, 22,(24), 5506-5507.< p>

K Guo.; J Wang.; V Andres.; RC Smith. MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation. Mol. Cell. Biol. 1995, 15,(7), 3823-3829.< p>

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H319

Causes serious eye irritation.

Sicherheitshinweise: P280i-P264-P305+P351+P338-P337+P313

Wear eye/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
Yes

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