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NP-40 lysis buffer, low salt - J60902 - Alfa Aesar

J60902 NP-40 lysis buffer, low salt

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
250ml 40,17
500ml 64,27
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NP-40 lysis buffer, low salt

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Fully misicible in water.

Anwendungen

Lysis buffer for extracting proteins from cells. NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay, ELISA. This lysis buffer can be used to prepare cell lysates for western blots or immunoprecipitation. It can also be used as a wash buffer for immunoprecipitation reactions.

Bemerkungen

This buffer contains: 50mM Tris-HCl (pH 7.4), 1% NP-40, and 5mM EDTA. To use, incubate cells in 5 volumes of buffer on ice for 10-30 minutes.

Literaturverweise

K Guo, J Wang,; V Andrés,; R C Smith and K Walsh. MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation.. Mol. Cell. Biol.1995, 15 (7), 3823-3829.

J L Taupin,; Q Tian,; N Kedersha,; M Robertson,; P Anderson The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death.. PNAS. year of publication , 92 (5) , 1629-1633.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
Yes

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