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91-95-2 - 3,3'-Diaminobenzidine, tablets - J60972 - Alfa Aesar

J60972 3,3'-Diaminobenzidine, tablets

CAS-Nummer
91-95-2
Synonyme

Größe Preis ($) Menge Verfügbarkeit
50each 110,21
100each 197,16
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3,3'-Diaminobenzidine, tablets

MDL
MFCD00007725
EINECS
202-110-6

Chemische Eigenschaften

Formel
C12H14N4
Molmasse
214.27
Form
Tablets
Sensitivität
Light Sensitive
Löslichkeit
Soluble in water (partly).

Anwendungen

3,3'-Diaminobenzidine is used to detect fingerprints in blood. 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Slides stained with DAB may be coverslipped in the traditional manner and stored for future reference. Many enhancement methods have been reported for DAB involving different buffer conditions, the addition of metal ions and post-treatment applications. DAB has been used in immunohistochemical staining of nucleic acids and proteins. Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain. 3, 3?-diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of carcinoembryonic antigen (CEA) in human serum.

Bemerkungen

Each tablet contains 5mg of DAB, each tablet weighs 240mg. One tablet will yield 10 ml of substrate solution, enough to effectively detect 50cm² of membrane or about 20 slides. Insoluble product. Reaction Condition: Dissolve one 5mg tablet in 10 ml of 50mM Tris, 0.1M NaCl, pH 7.6 and 0.01% hydrogen peroxide. The solution may be filtered through a 0.2µm filter immediately prior to use if necessary. The presence of nickel or cobalt increases sensitivity.

Literaturverweise

Zs. Liposits,; Gy. Sétaló, B. Flerkó.Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain . Neuroscience. 1984, 13 (2), 513-525.

Volker Herzog,; H.Dariush Fahimi. A new sensitive colorimetric assay for peroxidase using 3,3?-diaminobenzidine as hydrogen donor . Analytical Biochemistry. 1973 , 55 (2), 554-562.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H350-H341

May cause cancer. Suspected of causing genetic defects.

Sicherheitshinweise: P201-P281-P202-P308+P313-P405-P501a

Obtain special instructions before use. Use personal protective equipment as required. Do not handle until all safety precautions have been read and understood. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Beilstein
1212988
Harmonized Tariff Code
2921.59
TSCA
Yes
RTECS
DV8750000

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