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77-86-1 - TRIS, 1.0M buffer soln., pH 8.6 - J61066 - Alfa Aesar

J61066 TRIS, 1.0M buffer soln., pH 8.6

CAS-Nummer
77-86-1
Synonyme

TRIS, 1.0M buffer soln., pH 8.6

MDL
MFCD00004679

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Fully miscible in water. Soluble in ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), chloroform (0.05 mg/ml), and alkaline solution.

Anwendungen

TRIS is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. It has a pKa of 8.06 and is very useful in the molecular biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

Bemerkungen

Stable under recommended storage conditions. Incompatible with oxidizing agents.

Literaturverweise

Steven P.Fling; Dale S.Gregerson. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Analytical Biochemistry. 1986, 155,(1), 83-88.

 

R.T.Irvin; T.J.MacAlister; J.W.Costerton. Tris(hydroxymethyl)aminomethane buffer modification of Escherichia coli outer membrane permeability. J Bacteriol. 1981, 145,(3), 1397-1403.

Weitere Referenzen

Harmonized Tariff Code
2922.19
TSCA
Yes

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