BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%. It is also useful for saturating excess protein-binding sites on the membranes and microplates in immunoassays.
Wittmann, K.; Dietl, S.; Ludwig, N.; Berberich, O.; Hoefner, C.; Storck, K.; Blunk, T.; Bauer-Kreisel, P. Engineering Vascularized Adipose Tissue Using the Stromal-Vascular Fraction and Fibrin Hydrogels. Tissue Eng. Part A 2015, 21 (7-8), 1343-1353.
Reichard, A. C.; Cheemarla, N. R.; Bigley, N. J. SOCS1/3 Expression Levels in HSV-1-Infected, Cytokine-Polarized and -Unpolarized Macrophages. J. Interferon Cytokine Res. 2015, 35 (1), 32-41.