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HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.
Sheila Timmons; Jack Hawiger. Separation of human platelets from plasma proteins including factor VIIIVWF by a combined albumin gradient-gel filtration method using hepes buffer. Thrombosis Research. 1978, 12,(2), 297-306.
Raymond Wai-Yin Sun; Rong Chen; Nancy P.-Y. Chung; Chi-Ming Ho; Chen-Lung Steve Lin; Chi-Ming Che. Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Chem. Commun. 2005, (40), 5059-5061.