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54827-17-7 - 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity - TMB soluble reagent, high sensitivity - J61325 - Alfa Aesar

J61325 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

CAS-Nummer
54827-17-7
Synonyme
TMB soluble reagent, high sensitivity

Größe Preis ($) Menge Verfügbarkeit
125ml 112,50
500ml 183,00
1000ml 288,00
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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

MDL
MFCD00007748
EINECS
259-364-6

Chemische Eigenschaften

Formel
C16H20N2
Molmasse
240.35
Form
Liquid
Löslichkeit
Insoluble in water. Soluble in organic solvents such as DMSO, 96 % Ethanol, Acetone, Chloroform, Toluene.

Anwendungen

TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Bemerkungen

The TMB solution has similar sensitivity levels to chemiluminescent detection reagents; it can detect as little as 0.15 ng. The TMB solution provided is enough for at least 25 mini-gel sized (10 X 10 cm) blots. Store at 2-8°C. Keep the substrate out of direct sunlight.

Literaturverweise

T Giersch. A new monoclonal antibody for the sensitive detection of atrazine with immunoassay in microtiter plate and dipstick format. J. Agric. Food Chem. 1993, 41, (6), 1006-1011.

AR Johnsen.; OS Jacobsen. A quick and sensitive method for the quantification of peroxidase activity of organic surface soil from forests. Soil Biology and Biochemistry. 2008, 40, (3), 814-821.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H312-H332-H370

Harmful in contact with skin. Harmful if inhaled. Causes damage to organs.

Sicherheitshinweise: P260-P280h-P304+P340-P307+P311a-P405-P501a

Do not breathe dust/fume/gas/mist/vapours/spray. Wear protective gloves/clothing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF exposed: Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Beilstein
2808541
Gefahrenklasse
6.1
Verpackungsgruppe
III
Harmonized Tariff Code
2921.59
TSCA
Yes

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