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Apo-Transferrin is also an important component used for the cultivation of mammalian cells in-vitro because it acts as a transport factor for defined culture media. This property is crucial for long-term mammalian cell growth in-vitro since ATF operates in media by binding to contaminating metal ions. Some other common uses for Apo-Transferrin are affinity purification and receptor mediated transfection of DNA-like molecules into cells.
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Y Zhang.; WM Pardridge. Rapid transferrin efflux from brain to blood across the blood-brain barrier. Journal of neurochemistry.2001, 76, (5), 1597-1600.