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9001-75-6 - Pepsin, porcine stomach - EC 3.4.23.1 - Pepsin A - J61679 - Alfa Aesar

J61679 Pepsin, porcine stomach

CAS-Nummer
9001-75-6
Synonyme
EC 3.4.23.1
Pepsin A

Größe Preis ($) Menge Verfügbarkeit
1g 115,36
5g 188,49
10g 300,00
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Pepsin, porcine stomach

MDL
MFCD00081840
EINECS
232-629-3

Chemische Eigenschaften

Form
Powder
Löslichkeit
Soluble in water and dilute aqueous buffers

Anwendungen

Pepsin is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. has been used in the digestion of crude wheat gliadin. It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue. It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues. The enzyme has also been used in the preparation of F(ab)2 fragment from IgG.

Bemerkungen

Minimum 2,500 units/mg. One unit increases absorbance at 280nm by 0.001 per minute at 37 C and pH 2.0, measured as TCA soluble products from denatured hemoglobin

Literaturverweise

TJ McDonald.; G Nilsson.; M Vagne.; M Ghatei. A gastrin releasing peptide from the porcine nonantral gastric tissue. Gut . < span>1978, 19, (2), 767-774.

J Jancarik.; SH Kim. Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst. < span>1978, 24, 409-411.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Sicherheitshinweise: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Merck
14,7146
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
SC6132000

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