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RIPA buffer with EGTA - J61951 - Alfa Aesar

J61951 RIPA buffer with EGTA

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
250ml 64,27
500ml 112,27
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RIPA buffer with EGTA

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Fully miscible in water.

Anwendungen

RIPA buffer with EGTA is used in general cell-tissue lysis and subcellular orgnalle or molecular fractionation, including radio Immunoprecipitation. It is used for extracting proteins from cells. It is used for nuclear membrane disruption for nuclear extracts. It helps in disrupting protein-protein interactions.

Bemerkungen

50mM Tris-HCl (pH 7.4), 150mM NaCl 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS with 1mM EGTA.

Literaturverweise

Robert Eskes; Solange Desagher; Bruno Antonsson and Jean-Claude Martinou. Bid Induces the Oligomerization and Insertion of Bax into the Outer Mitochondrial Membrane. Mol. Cell. Biol. 2000, 20, (3), 929-935.

Ramakrishnapillai V. Omkumar; Melinda J. Kiely; Alan J. Rosenstein; Kyung-Tai Min and Mary B. Kennedy. Identification of a Phosphorylation Site for Calcium/Calmodulindependent Protein Kinase II in the NR2B Subunit of the N-Methyl-D-aspartate Receptor. Journal of Biological Chemistry. 2000, 271, 31670-31678.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H319

Causes serious eye irritation.

Sicherheitshinweise: P280-P264-P305+P351+P338-P337+P313

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
Yes

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