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Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis, inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids.
Betzel C.; Singh TP.; Visanji M.; Peters K.; Fittkau S.; Saenger W.; Wilson KS. Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution. J. Biol. Chem. 1993, 268, (21), 15854-8.
Müller A.; Hinrichs W.; Wolf WM.; Saenger W. Crystal structure of calcium-free proteinase K at 1.5-A resolution. J. Biol. Chem. 1994, 269, (37), 23108-11.
Gefahrenhinweise (EU): H315-H319-H334-H335
Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.