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39450-01-6 - Proteinase K, Tritirachium album limber - J62051 - Alfa Aesar

J62051 Proteinase K, Tritirachium album limber

CAS-Nummer
39450-01-6
Synonyme

Größe Preis ($) Menge Verfügbarkeit
25mg 49,75
100mg 106,09
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Proteinase K, Tritirachium album limber

MDL
MFCD00132129
EINECS
254-457-8

Chemische Eigenschaften

Form
Lyophilized powder, 28.9 kDa
Löslichkeit
It is fully miscible with water.

Anwendungen

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis, inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids.

Bemerkungen

Minimum 20 units per mg dry weight. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37 C and pH 7.5, using urea denatured hemoglobin as the substrate

Literaturverweise

Betzel C.; Singh TP.; Visanji M.; Peters K.; Fittkau S.; Saenger W.; Wilson KS. Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution.   J. Biol. Chem. 1993, 268, (21), 15854-8.

Müller A.; Hinrichs W.; Wolf WM.; Saenger W. Crystal structure of calcium-free proteinase K at 1.5-A resolution. J. Biol. Chem. 1994, 269, (37), 23108-11.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Sicherheitshinweise: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Harmonized Tariff Code
3507.90
TSCA
No

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