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1239-45-8 - Ethidium bromide soln., 10mg/ml - J62282 - Alfa Aesar

J62282 Ethidium bromide soln., 10mg/ml

CAS-Nummer
1239-45-8
Synonyme

Größe Preis ($) Menge Verfügbarkeit
10ml 21,90
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Ethidium bromide soln., 10mg/ml

MDL
MFCD00011724
EINECS
214-984-6

Chemische Eigenschaften

Formel
C21H20BrN3
Molmasse
394.32
Form
Liquid
Löslichkeit
Soluble in water (10 mg/mL).

Anwendungen

Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Bemerkungen

Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Literaturverweise

S P Moore and B M Sutherland. A densitometric nondestructive microassay for DNA quantitation. Analytical Biochemistry. 1985, 144(1), 15-19.

A R El Hamalawi et. al. The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. Analytical Biochemistry. 1975, 67(2), 384-391.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H341-H333

Suspected of causing genetic defects. May be harmful if inhaled.

Sicherheitshinweise: P201-P281-P202-P304+P312-P308+P313-P405-P501a

Obtain special instructions before use. Use personal protective equipment as required. Do not handle until all safety precautions have been read and understood. IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Merck
14,4731
Gefahrenklasse
6.1
Verpackungsgruppe
III
Harmonized Tariff Code
2933.99
TSCA
No
RTECS
SF7950000

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