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Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.
Meyer, D.L., et al. Reduced antibody response to streptavidin through site-directed mutagenesis. Protein Sci. 2001, 10, (3), 491-503.
Rusakiewicz, S., et al. Soluble HLA/peptide monomers cross-linked with co-stimulatory antibodies onto a streptavidin core molecule efficiently stimulate antigen-specific T cell responses. span class="italictxt"> Cancer Immunol. Immunother. 2009, 58, (9), 1459-1470.