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RIPA buffer (5X) - J62524 - Alfa Aesar

J62524 RIPA buffer (5X)


Größe Preis ($) Menge Verfügbarkeit
50ml 56,20
100ml 96,40
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RIPA buffer (5X)


Chemische Eigenschaften

It is soluble in water.


RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation.


250mM Tris-HCl (pH 7.4), 750mM NaCl, 5% NP-40, 2.5% sodium deoxycholate,0.5% SDS.


MS Mccoy.; CI Bargmann. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 1984, 4, (8), 1577-1582.

T Dorfman.; A Bukovsky.; A Ohagen.; S Höglund. Functional domains of the capsid protein of human immunodeficiency virus type 1. J. Virol. 1994, 68, (12), 8180-8187.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H318

Causes serious eye damage.

Sicherheitshinweise: P280i-P305+P351+P338-P310a

Wear eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor

Weitere Referenzen

Harmonized Tariff Code


  • A15797

    1,4-Dithio-DL-threitol, 98%
  • B24026

    4-(Trifluoromethylthio)benzyl alcohol, 97%
  • J60143

    NP-40 lysis buffer with glycerol (2X)
  • J60568

    PIPES lysis buffer with NP-40 (2X)
  • J60015

    Laemmli SDS sample buffer, reducing (4X)

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