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RIPA buffer (5X) - J62524 - Alfa Aesar

J62524 RIPA buffer (5X)

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
50ml 56,20
100ml 96,40
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RIPA buffer (5X)

MDL
MFCD02100484

Chemische Eigenschaften

Form
Liquid
Löslichkeit
It is soluble in water.

Anwendungen

RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation.

Bemerkungen

250mM Tris-HCl (pH 7.4), 750mM NaCl, 5% NP-40, 2.5% sodium deoxycholate,0.5% SDS.

Literaturverweise

MS Mccoy.; CI Bargmann. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 1984, 4, (8), 1577-1582.

T Dorfman.; A Bukovsky.; A Ohagen.; S Höglund. Functional domains of the capsid protein of human immunodeficiency virus type 1. J. Virol. 1994, 68, (12), 8180-8187.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H318

Causes serious eye damage.

Sicherheitshinweise: P280-P305+P351+P338-P310

Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
Yes

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Kürzlich angesehen

Chemikalien

Life science

Metalle und Stoffe

Katalysatoren

Forschungs- und Laborbedarf