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Nitrocellulose stripping buffer (10X) - J62541 - Alfa Aesar

J62541 Nitrocellulose stripping buffer (10X)

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
250ml 39,80
500ml 63,60
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Nitrocellulose stripping buffer (10X)

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Not miscible or difficult to mix in water.

Anwendungen

Ideal for removal of secondary antibody after detection of highly abundant targets. Nitrocellulose Stripping Buffer may perform well with nitrocellulose membranes or antibody-dye conjugates. It is also employed for nucleic acid and protein related blotting works.

Bemerkungen

This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane dilute buffer to 1X in water. Warm solution to 50-70°C. Perform 2 to 3 changes (15 minutes) of the warmed 1X stripping buffer.

Literaturverweise

Donald W. MacGlashan Jr. Endocytosis, recycling, and degradation of unoccupied FcεRI in human basophils.Journal of Leukocyte Biology.,2007,82(4), 1003-1010.

Francisco García-Carrizo, et al. Leptin Effect on Acetylation and Phosphorylation of PGC1α in Muscle Cells Associated With AMPK and Akt Activation in High-Glucose Medium.Journal of Cellular Physiology.,2015,1-30.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
Yes

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