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TE buffer, pH 8.1, RNAse free - J62745 - Alfa Aesar

J62745 TE buffer, pH 8.1, RNAse free

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
125ml 39,80
250ml 71,60
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TE buffer, pH 8.1, RNAse free

MDL
MFCD00236359

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Not miscible or difficult to mix in water.

Anwendungen

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

Bemerkungen

200mM Tris-HCl with 20mM EDTA

For a final concentration of EDTA as 0.1 mM, dilute TE buffer 200-fold with RNase and DNase free water.

Literaturverweise

H Ito, et al. Transformation of intact yeast cells treated with alkali cations.J. Bacteriol.,1983, 153, (1), 163-168.

Hamilton O. Smith and Max L. Birnstiel. A simple method for DNA restriction site mapping. Nucl. Acids Res.,1976, 3, (9), 2387-2398.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
Yes

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