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145224-94-8 - MES, 1.0M buffer soln., pH 7.5 - J62752 - Alfa Aesar

J62752 MES, 1.0M buffer soln., pH 7.5

CAS-Nummer
145224-94-8
Synonyme

Größe Preis ($) Menge Verfügbarkeit
250ml 44,55
500ml 82,00
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MES, 1.0M buffer soln., pH 7.5

MDL
MFCD00283848

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Soluble in water (0.5 M at 20°C).

Anwendungen

MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.

Bemerkungen

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Literaturverweise

BG Bugbee.; FB Salisbury. An evaluation of MES (2 (N-Morpholino) ethanesulfonic acid) and amberlite IRC-50 as pH buffers for nutrient solution studies. Journal of plant nutrition. 1985, 8, ( 7), 567-583.

MJ Alowitz.; MM Scherer . Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology. 2002, 36, (3), 299-306.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Sicherheitshinweise: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Harmonized Tariff Code
2934.99
TSCA
No

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