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DNA marker, high range, 1,000 Base Pair Ladder - J63178 - Alfa Aesar

J63178 DNA marker, high range, 1,000 Base Pair Ladder

CAS-Nummer
Synonyme

Größe Preis ($) Menge Verfügbarkeit
1ml 72,31
2ml 106,40
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DNA marker, high range, 1,000 Base Pair Ladder

Chemische Eigenschaften

Form
Liquid
Löslichkeit
Soluble in 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl.

Anwendungen

DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. It is commonly used in the studies of plasmid DNA and PCR products. 1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during electrophoresis. 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In NA electrophoresis, five ?l of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.

Bemerkungen

Contains 8 bands from 1,000-10,000 base pairs: 1000bp increments from 1,000-6,000bp, 8,000 and 10,000bp. Recommended load volume is 20µl.

Literaturverweise

T Asahida.; T Kobayashi.; K Saitoh.; I Nakayama. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fisheries Science. 199662 (5), 727-730.

J Welsh.; M McClelland. Fingerprinting genomes using PCR with arbitrary primers. Nucleic acids research. 199018 (24), 7213-7218.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H319

Causes serious eye irritation.

Sicherheitshinweise: P280-P264-P305+P351+P338-P337+P313

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Weitere Referenzen

Harmonized Tariff Code
3822.00
TSCA
No

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