I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.
Tween-20 is a non-ionic surfactant that is useful for solubilizing membrane proteins during isolation. Tween-20 is also added to buffers for immunohistochemistry. Tween-20 may also help to prevent non-specific antibody binding and function as a blocking agent in immunoblotting assays. It has been used in preextraction of membranes to remove peripheral proteins.
Nicole Schaeren-Wiemers.; Andrea Gerfin-Moser. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. Histochemistry.1993 , 100, (6), 431-440.
Eric Lecuyer.; Neela Parthasarathy.; Henry M. Krause. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues. Drosophila.2008, 420, 289-302.