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39450-01-6 - Proteinase K, Ready-to-Use soln. - J63710 - Alfa Aesar

J63710 Proteinase K, Ready-to-Use soln.

CAS-Nummer
39450-01-6
Synonyme

Größe Preis ($) Menge Verfügbarkeit
5ml 244,11
25ml 748,80
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Proteinase K, Ready-to-Use soln.

MDL
MFCD00132129
EINECS
254-457-8

Chemische Eigenschaften

Form
Liquid, 28.9 kDa
Löslichkeit
Miscible with water.

Anwendungen

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

Bemerkungen

Minimum 400 units per ml. Protein concentration is 20 mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. RNase and DNase free. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C and pH 7.5, using urea denatured hemoglobin as the substrate.Incompatible with strong oxidizing agents. Store in a cool place.

Literaturverweise

Fränzel, B.; Fischer, F.; Steegborn, C.; Wolters, D. A. Proteinase K improves quantitative acylation studies. Proteomics 2015, 15 (1), 44-47.

Shishova, K. V.; Khodarovich, Y. M.; Lavrentyeva, E. A.; Zatsepina, O. V. Analysis of the localization of fibrillarin and sites of pre-rRNA synthesis in the nucleolus-like bodies of mouse GV oocytes after mild treatment with proteinase K. Russ. J. Dev. Biol. 2015, 46 (3), 127-136.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H334-H319

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes serious eye irritation.

Sicherheitshinweise: P285-P261-P280-P264-P305+P351+P338-P342+P311-P304+P341-P337+P313-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. If eye irritation persists: Get medical advice/attention. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Harmonized Tariff Code
3507.90
TSCA
No

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