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Blot blocking buffer is used to block non-specific binding site for antibodies on the membrane. It may be used for multiple applications, including as a diluent for antibody.
R L Ashley.; J Militoni, F Lee.; A Nahmias.; L Corey. Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera.J. Clin. Microbiol.. 1988, 26 (4), 662-667 .