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9002-07-7 - Trypsin 1:250, porcine pancreas - EC 3.4.21.4 - J63993 - Alfa Aesar

J63993 Trypsin 1:250, porcine pancreas

CAS-Nummer
9002-07-7
Synonyme
EC 3.4.21.4

Größe Preis ($) Menge Verfügbarkeit
10g 123,00
50g 210,00
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Trypsin 1:250, porcine pancreas

MDL
MFCD01775455
EINECS
232-650-8

Chemische Eigenschaften

Form
Powder
Löslichkeit
Soluble in Hank's Balanced Salt Solution (35 mg/ml), HCl (1 mM), and water. Insoluble in clycerol and alcohol.

Anwendungen

Trypsin 1:250 is used in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It has also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Bemerkungen

Low endotoxin. Minimum 225 units/mg. One unit causes a decrease in absorbance at 253 nm of 0.003 per minute at 25C.

Literaturverweise

Paul P.Geurink; Wouter A van der Linden; Anne C.Mirabella; Nerea Gallastegui; Gerjan de Bruin; Annet E.M.Blom; Mathias J.Voges; Elliot D.Mock; Bogdan I.Florea; Gijs A van der Marel; Christoph Driessen; Mario van der Stelt; Michael Groll; Herman S.Overkleeft; Alexei F.Kisselev. Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites. Journal of medicinal and pharmaceutical chemistry. 2013, 56,(3), 1262-1275.

 

Simone A.Beckham; Jason Brouwer; Anna Roth; Die Wang; Anthony J.Sadler; Matthias John; Kerstin Jahn-Hofmann; Bryan R.G.Williams; Jacqueline A.Wilce; Matthew C.J.Wilce. Conformational rearrangements of RIG-I receptor on formation of a multiprotein:dsRNA assembly. Nucleic Acids Research. 2013, 41,(5), 3436-3445.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Sicherheitshinweise: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Merck
14,9795
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
YN5075000

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