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5-Bromo-4-chloro-3-indolyl phosphate disodium salt, is used for the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
Chuanmin Ruan.; Kefeng Zeng.; Oomman K Varghese.; Craig A Grimes. A magnetoelastic bioaffinity-based sensor for avidin. Biosensors and Bioelectronics. 2004, 19, (12), 1695-1701.
Chuanmin Ruan.; Liju Yang.; Yanbin Li. Immunobiosensor Chips for Detection of Escherichia coli O157:H7 Using Electrochemical Impedance Spectroscopy. Anal. Chem. 2002, 74, (18), 4814-4820.