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593-84-0 - Guanidine thiocyanate, Molecular Biology Grade - Guanidine rhodanide - J65104 - Alfa Aesar

J65104 Guanidine thiocyanate, Molecular Biology Grade

CAS-Nummer
593-84-0
Synonyme
Guanidine rhodanide

Größe Preis ($) Menge Verfügbarkeit
50g 73,80
250g 155,00
500g 263,00
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Guanidine thiocyanate, Molecular Biology Grade

MDL
MFCD00013027
EINECS
209-812-1

Chemische Eigenschaften

Formel
CH5N3.CHNS
Molmasse
118.16
Form
Crystalline solid
Schmelzpunkt
118-122°
Dichte
1.29
Sensitivität
Light Sensitive
Löslichkeit
Soluble in water (1420 g/L) at 20°C, and ethanol (5%).

Anwendungen

Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.

Bemerkungen

Light Sensitive. Keep container tightly closed. Store away from oxidizing agents.

Literaturverweise

Gregory J. Wadsworth.; Margaret G. Redinbaugh.; John G. Scandalios. A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis . Analytical Biochemistry. 1988, 172, (1), 279-283.

John M. Chirgwin.; Alan E. Przybyla.; Raymond J. MacDonald.; William J. Rutter. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979, 18, (24), 5294-5299.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H302-H312-H332-H402-H412

Harmful if swallowed. Harmful in contact with skin. Harmful if inhaled. Harmful to aquatic life. Harmful to aquatic life with long lasting effects.

Sicherheitshinweise: P261-P273-P280-P264-P304+P340-P301+P312-P312-P363-P322-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Avoid release to the environment. Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Wash contaminated clothing before reuse. Specific measures (see label). Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Beilstein
3563461
Harmonized Tariff Code
2925.29
TSCA
Yes
RTECS
XL1225000

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