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Carboxypeptidase Y from yeast is used for C-terminal sequencing of proteins. Also for C-terminal modification of proteins and peptides. Due to its amidase action, it is applied to the sequence analysis of peptides having amidated COOH-terminal groups such as oxytocin and vasopressin.
Tom Stevens.; Brent Esmon.; Randy Schekman. Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole. Cell. 1982, 30, (2), 439-448.
Heidrun Matern.; Heinrich Betz.; Helmut Holzer. Compartmentation of inhibitors of proteinases A and B and carboxypeptidase Y in yeast. Biochemical and Biophysical Research Communications. 1974, 60, (3), 1051-1057.
Gefahrenhinweise (EU): H315-H319-H334-H335
Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.