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50-01-1 - Guanidine hydrochloride, Molecular Biology Grade - J65661 - Alfa Aesar

J65661 Guanidine hydrochloride, Molecular Biology Grade

CAS-Nummer
50-01-1
Synonyme

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Guanidine hydrochloride, Molecular Biology Grade

MDL
MFCD00013026
EINECS
200-002-3

Chemische Eigenschaften

Formel
CH5N3•HCl
Molmasse
95.53
Form
Crystalline solid
Schmelzpunkt
178-185°
Dichte
1.34
Sensitivität
Hygroscopic
Löslichkeit
Freely soluble in water

Anwendungen

Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Bemerkungen

Protect from heat. Store away from oxidizing agents.

Literaturverweise

Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.

Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment:? Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H302-H315-H319

Harmful if swallowed. Causes skin irritation. Causes serious eye irritation.

Sicherheitshinweise: P280a-P305+P351+P338-P301+P312a-P321-P332+P313-P501a

Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF SWALLOWED: Specific treatment (see label). If skin irritation occurs: Get medical advice/attention. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Merck
14,4562
Beilstein
3591990
Harmonized Tariff Code
2925.29
TSCA
Yes
RTECS
MF4300000

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