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75621-03-3 - CHAPS, Electrophoresis Grade - 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate - J67359 - Alfa Aesar

J67359 CHAPS, Electrophoresis Grade

CAS-Nummer
75621-03-3
Synonyme
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

Größe Preis ($) Menge Verfügbarkeit
5g 86,57
10g 175,88
25g 429,00
Zum Warenkorb hinzufügen Zur Angebotsanfrage hinzufügen Artikel anzeigen

CHAPS, Electrophoresis Grade

MDL
MFCD00012116

Chemische Eigenschaften

Formel
C32H58N2O7S
Molmasse
614.88
Form
Powder
Schmelzpunkt
156-158°
Sensitivität
Hygroscopic
Löslichkeit
Soluble in water (10 mg/ml). Soluble in DMF and hot methanol.

Anwendungen

CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.

Bemerkungen

Hygroscopic. Store away from water/moisture and oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store under inert gas.

Literaturverweise

Jacob Anglister, et. al. Isotope-edited multidimensional NMR of calcineurin B in the presence of the non-deuterated detergent CHAPS.J. Biomol. NMR.,1993,3(1), 121-126.

F. Anne Stephenson and Richard W. Olsen. Solubilization by CHAPS Detergent of Barbiturate-Enhanced Benzodiazepine-GABA Receptor Complex.J. Neurochem.,1982,39(6), 1579-1586.

GHS Gefahren- und Sicherheitshinweise

Gefahrenhinweise (EU): H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Sicherheitshinweise: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Weitere Referenzen

Harmonized Tariff Code
2924.29
TSCA
No

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