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Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.
Helene Breitschopf.; Gerda Suchanek.; Robert M. Gould.; David R. Colman.; Hans Lassmann. In situ hybridization with digoxigenin-labeled probes: sensitive and reliable detection method applied to myelinating rat brain.Acta Neuropathologica. 1992, 84 (6),581-587 .
Gefahrenhinweise (EU): H302-H351
Harmful if swallowed. Suspected of causing cancer.
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