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4-Methylumbelliferone is used in the determination of nitric acid and as a standard for fluorometric determination of enzyme activity. It is also used in bile therapy.
Standard for fluorimetric determination of enzyme activity: Anal. Biochem., 54, 40; 55, 85 (1973)./n
Franco, P. G.; Perez, M. J.; Aranda, C.; Adamo, A.; Silvestroff, L. Improving arylsulfatase activity determination in dried blood spots: Screening and diagnostic approaches for Maroteaux-Lamy syndrome (MPS VI). Clin. Chim. Acta 2015, 446, 86-92.
Kłucinska, K.; Jurczakowski, R.; Maksymiuk, K.; Michalska, A. Ultrasensitive 4-methylumbelliferone fluorimetric determination of water contents in aprotic solvents. Talanta 2015, 132, 392-397.
Hazard Statements: H303-H315-H319-H335
May be harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P280a-P305+P351+P338-P304+P340-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.