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Amido Black 10B is used as a histological, protein, and nucleic acid dye used in electrophoresis. Naphthol Blue Black is an important and commonly used industrial dye. This dye is also used in rapid staining of protein bands on nitrocellulose membranes such as Western blots. Naphthol Blue Black can be utilized as a nucleic acid stain in SDS-Page gels. Additionally this compound has been untilized in cell proliferation assays and histochemical studies. It is also used in criminal investigations to detect blood present with latent fingerprints. They are also used in paints, inks, plastics and leather.
Beat J.; Brüschweiler.;Simon Küng.; Daniel Bürgi.; Lorenz Muralt.; Erich Nyfeler.Identification of non-regulated aromatic amines of toxicological concern which can be cleaved from azo dyes used in clothing textiles. Regulatory Toxicology and Pharmacology. 2014, 69, (2), 263-272.
Sangkil Nam.;Paul G.; Tratnyek. Reduction of azo dyes with zero-valent iron. Water Research. 2000, 34, (6), 1837-1845.
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P280a-P305+P351+P338-P304+P340-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.