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Phthaldialdehyde is used as a fluorescent reagent for checking the assay of amines present in proteins, peptides by capillary electrophoresis and chromatography. It is also used for cholesterol assay in the picomole range as well as for fluorometric determination of histamine, histidine and other amino acids. Poly(phthalaldehyde) is obtained by the polymerization reaction which is used to make a photoresist. The nitrogen by o-phthaldialdehyde assay (NOPA) is a method used in winemaking process to measure the completion of fermentation.
Reagent for precolumn derivatization of amino acids: J. Chromat., 359, 231 (1986).
Dai, Z.; Wu, Z.; Jia, S.; Wu, G. Analysis of amino acid composition in proteins of animal tissues and foods as pre-column o-phthaldialdehyde derivatives by HPLC with fluorescence detection. J. Chromatogr. B 2014, 964, 116-127.
Kpan, G. K.; Dembele, A.; Yao, L. B.; Tiho, S. Dosage du glyphosate par HPLC après extraction et dérivation à lO-phthaldialdehyde (OPA). Int. J. Biol. Chem. Sci. 2015, 9 (3), 1384-1398.
Hazard Statements: H301-H314-H318-H317
Toxic if swallowed. Causes severe skin burns and eye damage. Causes serious eye damage. May cause an allergic skin reaction.
Precautionary Statements: P260u-P301+P310a-P303+P361+P353-P305+P351+P338-P405-P501a
Do not breathe dusts or mists. IF SWALLOWED: Immediately call a POISON CENTER/doctor IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.