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54827-17-7 - 3,3',5,5'-Tetramethylbenzidine, 98% - 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl - TMB - A13868 - Alfa Aesar

A13868 3,3',5,5'-Tetramethylbenzidine, 98%

CAS Number
54827-17-7
Synonyms
4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl
TMB

Size Price ($) Quantity Availability
1g 36.27
5g 121.28
25g 387.20
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3,3',5,5'-Tetramethylbenzidine, 98%

MDL
MFCD00007748
EINECS
259-364-6

Chemical Properties

Formula
C16H20N2
Formula Weight
240.35
Melting point
168-171°
Sensitivity
Moisture & Light Sensitive
Solubility
Soluble in ethyl acetate, ethanol, acetone, chloroform, toluene and dimethyl sulfoxide. Insoluble in water.

Applications

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

Notes

Light and moisture sensitive. Store in a cool place. Incompatible with metals, strong acids and strong oxidizing agents.

Literature References

Tang, S.; Chang, Y.; Chia, G. H.; Lee, H. K. Selective extraction and release using (EDTA-Ni)-layered double hydroxide coupled with catalytic oxidation of 3,3',5,5'-tetramethylbenzidine for sensitive detection of copper ion. Anal. Chim. Acta 2015, 885, 106-113.

Guo, Y.; Ma, Q.; Cao, F.; Zhao, Q.; Ji, X. Colorimetric detection of hypochlorite in tap water based on the oxidation of 3,3',5,5'-tetramethylbenzidine. Anal. Methods 2015, 7 (10), 4055-4058.

GHS Hazard and Precautionary Statements

Hazard Statements: H302-H315-H319-H335

Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P280-P305+P351+P338-P304+P340-P362-P301+P312-P312-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Beilstein
2808541
Harmonized Tariff Code
2921.59
TSCA
Yes
RTECS
DV2300000

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