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A13868 3,3',5,5'-Tetramethylbenzidine, 98%

CAS Number

Stock No. Size Price ($) Quantity Availability
A13868-03 1g 39.00
A13868-06 5g 136.00
A13868-14 25g 483.00
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3,3',5,5'-Tetramethylbenzidine, 98%


Chemical Properties

Formula Weight
Melting point
Storage & Sensitivity
Moisture Sensitive. Light Sensitive. Store under Nitrogen. Ambient temperatures.
Soluble in ethyl acetate, ethanol, acetone, chloroform, toluene and dimethyl sulfoxide. Insoluble in water.


Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.


Light and moisture sensitive. Store in a cool place. Incompatible with metals, strong acids and strong oxidizing agents.

Literature References

Tang, S.; Chang, Y.; Chia, G. H.; Lee, H. K. Selective extraction and release using (EDTA-Ni)-layered double hydroxide coupled with catalytic oxidation of 3,3',5,5'-tetramethylbenzidine for sensitive detection of copper ion. Anal. Chim. Acta 2015, 885, 106-113.

Guo, Y.; Ma, Q.; Cao, F.; Zhao, Q.; Ji, X. Colorimetric detection of hypochlorite in tap water based on the oxidation of 3,3',5,5'-tetramethylbenzidine. Anal. Methods 2015, 7 (10), 4055-4058.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H332-H335-H341

Causes skin irritation. Causes serious eye irritation. Harmful if inhaled. May cause respiratory irritation. Suspected of causing genetic defects.

Precautionary Statements: P201-P202-P261-P264-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P308+P313-P312-P330-P332+P313-P337+P313-P362-P501c

Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Avoid breathing dust/fume/gas/mist/vapours/spray. Wash thoroughly after handling. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF exposed or concerned: Get medical advice/attention. Rinse mouth. If skin irritation occurs: If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

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