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69-78-3 - 5,5'-Dithiobis(2-nitrobenzoic acid), 99% - Bis(3-carboxy-4-nitrophenyl) disulfide - 3-Carboxy-4-nitrophenyl disulfide - A14331 - Alfa Aesar

A14331 5,5'-Dithiobis(2-nitrobenzoic acid), 99%

CAS Number
Bis(3-carboxy-4-nitrophenyl) disulfide
3-Carboxy-4-nitrophenyl disulfide

Size Price ($) Quantity Availability
1g 20.61
5g 57.06
25g 217.80
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5,5'-Dithiobis(2-nitrobenzoic acid), 99%


Chemical Properties

Formula Weight
Melting point
ca 240° dec.
Soluble in water and ethanol.


Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.


Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.

Literature References

Reacts with free thiol groups to form a highly colored (yellow) thiolate anion: Arch. Biochem. Biophys., 82, 70 (1959); used for quantitative determination of thiols. Also used in chromogenic assay of acetylcholinesterase: Enzyme Assays, R. Eisenthal and M. J. Danson, Eds., OUP, Oxford (1992), p81.


Titi, H. M.; Nandi, G.; Thakuria, R.; Goldberg, I. Coordination polymers of 5,5'-dithiobis(2-nitrobenzoic acid): Synthesis, structure and topology. Inorg. Chim. Acta 2015, 426, 55-63.

Li, Z. G. Chapter Six - Quantification of Hydrogen Sulfide Concentration Using Methylene Blue and 5,5'-Dithiobis(2-Nitrobenzoic Acid) Methods in Plants. Methods Enzymol. 2015, 554, 101-110.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

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