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A14331 5,5'-Dithiobis(2-nitrobenzoic acid), 99%

CAS Number
69-78-3
Synonyms
Bis(3-carboxy-4-nitrophenyl) disulfide
3-Carboxy-4-nitrophenyl disulfide

Stock No. Size Price ($) Quantity Availability
A14331-03 1g 23.90
A14331-06 5g 62.10
A14331-14 25g 254.00
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5,5'-Dithiobis(2-nitrobenzoic acid), 99%

MDL
MFCD00007140
EINECS
200-714-4

Chemical Properties

Formula
C14H8N2O8S2
Formula Weight
396.36
Melting point
ca 240° dec.
Storage & Sensitivity
Ambient temperatures.
Solubility
Soluble in water and ethanol.

Applications

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

Notes

Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.

Literature References

Reacts with free thiol groups to form a highly colored (yellow) thiolate anion: Arch. Biochem. Biophys., 82, 70 (1959); used for quantitative determination of thiols. Also used in chromogenic assay of acetylcholinesterase: Enzyme Assays, R. Eisenthal and M. J. Danson, Eds., OUP, Oxford (1992), p81.

/n

Titi, H. M.; Nandi, G.; Thakuria, R.; Goldberg, I. Coordination polymers of 5,5'-dithiobis(2-nitrobenzoic acid): Synthesis, structure and topology. Inorg. Chim. Acta 2015, 426, 55-63.

Li, Z. G. Chapter Six - Quantification of Hydrogen Sulfide Concentration Using Methylene Blue and 5,5'-Dithiobis(2-Nitrobenzoic Acid) Methods in Plants. Methods Enzymol. 2015, 554, 101-110.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P337+P313-P362-P501c

Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Beilstein
1896821
Harmonized Tariff Code
2930.90
TSCA
Yes
RTECS
DG9650000

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