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HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
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Taha, M.; Khoiroh, I.; Lee, M. J. Phase behavior and molecular dynamics simulation studies of new aqueous two-phase separation systems induced by HEPES buffer. J. Phys. Chem. B 2013, 117, (2), 563-582.