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Bicine is widely utilized as a buffering agent for low temperature work. It is used in kinetic study of a quaternary transition-state analogue complex of creatine kinase reaction. It is employed in the thin layer ion exchange chromatography method for protein resolution. It is employed in serum guanase determination by the preparation of stable substrate solution.
Biological buffer, pK a = 8.35 at 20 o : Biochemistry, 5, 467 (1966).
Cowan, E. A.; Oldham, C. D.; May, S. W. Identification of a thioselenurane intermediate in the reaction between phenylaminoalkyl selenoxides and glutathione. Arch. Biochem. Biophys. 2011, 506 (2), 201-207.
Tainaka, K.; Kubota, S. I.; Suyama, T. Q.; Susaki, E. A.; Perrin, D.; Tadenuma, M. U.; Ukai, H.; Ueda, H. R. Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization. Cell 2014, 159 (4), 911-924.